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. 2023 Apr 14;14:1156219. doi: 10.3389/fimmu.2023.1156219

Table 1.

Primers used in this study.

Primers Sequence (5′ to 3′) Application
CD8α-F1 CAAAGTACACGGGTGGAGGAAGG 3′-RACE
CD8α-F2 TATATCTGGCATCGGTTCACAAAAT 3′-RACE
CD8α-R1 GGTGACTTTTTGGGATGATTTGTCA 5′-RACE
CD8α-R2 TCAGCCATGAGCGAGCAGGAGA 5′-RACE
CD8β-R1 TTGCGACTGATGTTCGGGTA 5′-RACE
CD8β-R2 GCGACTGATGTTCGGGTACT 5′-RACE
CD8α-F TCTCAAGTGCCCTCGTTCAC Verify full length
CD8α-R ATTGAGCTCTCAAGGGGTGC Verify full length
CD8β-F GGTCTTCAGCAAACAGCAACA Verify full length
CD8β-R TCCTTTCTCCACATGTTGGTCC Verify full length
rScCD8α-F CATGCCATGGCTAGCCTGCAGAGCACCCGTGTTGAAGAAGGTTCCAAAGTTGCCAT Plasmid construction
rScCD8α-R CGGGATCCTTACATGGCACAGGACAGCCCATCTTG Plasmid construction
APT CCAGACTCGTGGCTGATGCATTTTTTTTTTTTTTTT 3′-RACE
APG CCAGACTCGTGGCTGATGCAGGGGGGGGGGGGGG 5′-RACE
AP CCAGACTCGTGGCTGATGCA 5′- and 3′-RACE