Table 1.
Primers | Sequence (5′ to 3′) | Application |
---|---|---|
CD8α-F1 | CAAAGTACACGGGTGGAGGAAGG | 3′-RACE |
CD8α-F2 | TATATCTGGCATCGGTTCACAAAAT | 3′-RACE |
CD8α-R1 | GGTGACTTTTTGGGATGATTTGTCA | 5′-RACE |
CD8α-R2 | TCAGCCATGAGCGAGCAGGAGA | 5′-RACE |
CD8β-R1 | TTGCGACTGATGTTCGGGTA | 5′-RACE |
CD8β-R2 | GCGACTGATGTTCGGGTACT | 5′-RACE |
CD8α-F | TCTCAAGTGCCCTCGTTCAC | Verify full length |
CD8α-R | ATTGAGCTCTCAAGGGGTGC | Verify full length |
CD8β-F | GGTCTTCAGCAAACAGCAACA | Verify full length |
CD8β-R | TCCTTTCTCCACATGTTGGTCC | Verify full length |
rScCD8α-F | CATGCCATGGCTAGCCTGCAGAGCACCCGTGTTGAAGAAGGTTCCAAAGTTGCCAT | Plasmid construction |
rScCD8α-R | CGGGATCCTTACATGGCACAGGACAGCCCATCTTG | Plasmid construction |
APT | CCAGACTCGTGGCTGATGCATTTTTTTTTTTTTTTT | 3′-RACE |
APG | CCAGACTCGTGGCTGATGCAGGGGGGGGGGGGGG | 5′-RACE |
AP | CCAGACTCGTGGCTGATGCA | 5′- and 3′-RACE |