Skip to main content
. 2023 Jan 25;15(5):1255–1275. doi: 10.1016/j.jcmgh.2023.01.004

Figure 3.

Figure 3

Protective effect of antibiotics on hepatic I/R injury could be transferred to GF mice in FMT experiments. (A) Pattern diagram and protocol of FMT experiment. We transferred the fecal microbiota of WT (D-WT, n = 5) and ABX (D-ABX, n = 4) mice to GF mice (R-WT, n = 5; R-ABX, n = 4). One mouse in each of the D-ABX and R-ABX groups died unexpectedly. (B) α diversity comparison: Shannon index and Simpson index of the 4 groups. (C) β diversity of clustering analysis: PCA, PCoA, and NMDS analysis of the 4 groups at the OTU level. (D) Comparison of composition of gut microbiota at phylum level in the 4 groups. (E) H&E staining and Suzuki’s quantitative score of the R-WT and R-ABX groups. (F) TUNEL staining and semiquantitative analysis of R-WT and R-ABX groups. Scale bars, 100 μm. (G and H) Immunochemical staining of MPO (G) and cleaved caspase 3 (H) in the R-WT and R-ABX groups. (I) Serum ALT levels of R-WT and R-ABX groups. For all data, statistical comparisons between 2 groups were carried out by Student t test. For comparisons among 4 groups, the Kruskal–Wallis H test was used. P < .05 indicates significant differences.