Figure S5.
Changes in the BM niche microenvironment. (A and B) Analysis of the cytokines secreted in the BM fluid of Ctrl and BAtTA mice with (A) heatmap of unsupervised clustering of BM fluid cytokine levels after quantile normalization (Gen; genotype), and (B) detailed quantification of IL-6, MIP1α, Gal1, Gal3, LCN2, and GrB levels. BM fluids were obtained by flushing four long bones (femur and tibia) of each mouse with 200 µl media and were analyzed with the Raybiotech 200 mouse cytokine array (four independent experiments). The six cytokines constitutively secreted by BAtTA MPP3 are indicated on the left in A, and the full list of the cytokines differentially expressed in BAtTA vs. Ctrl BM fluids is provided in Table S5. (C) Representative images of GMP immunofluorescence staining in Ctrl, Gal3−/−, BAtTA, BAtTA:Gal3−/− BM. Dotted lines denote GMP clusters; Scale bar, 50 µm. Data are means ± SD, and significance was assessed by a two-tailed unpaired Student’s t test.