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. 2023 Apr 14;14:1104711. doi: 10.3389/fimmu.2023.1104711

Figure 7.

Figure 7

Role of type 1 IFN in regulation of infiltration of Tregs into the MIP treated TME: (A) The percentage of Tregs in IFNR1-/-/wild type mice in tumors treated with or without MIP. (B) Tumor bearing wild type and IFNR1-/- animals, 24 hours after the third dose of MIP, received an intravenous transfer of CFSE-labeled splenocytes of healthy wild type donor mice. Tumors were resected 18 hours after transfer, followed by flow cytometric analysis of intratumoral CFSE+ Treg cells. Proportion of CFSE+CD4+FoxP3+ Tregs within the total live CFSE+ cells was analyzed in the tumor of untreated/MIP treated mice. Flow cytometry contour plot is shown for one representative mouse per group. The bar graphs represent average of 5 mice per group. The results are expressed as mean ± SEM. Statistical analysis was performed using unpaired Student’s t test (*:p<0.05, **:p<0.01, ns, non-significant). Each experiment was repeated three times independent of each other.