Figure 8.

Importance of type 1 IFN in MIP mediated remodulation of percent frequency and effector function of intratumoral CD8⁺ T cells: (A) Percent frequency of intratumoral CD8⁺ T cells was determined in wild type and IFNR1^-/- tumor bearing mice treated with or without MIP. (B) Effect of MIP immunotherapy on proliferation of tumor-infiltrating CD8⁺ T cells in wild type and IFNR1^-/- mice. (C) In wildtype and IFNR1^-/- mice, effect of MIP therapy on effector function of intratumoral CD8 T cells was determined in terms of production of IFN-γ from CD8⁺ T cells. Flow cytometry contour plot is shown for one representative mouse per group. The bar graphs represent average of 5 mice per group. The results are expressed as means ± SEM. Statistical analysis was performed using unpaired non-parametric Student’s t test (*:p<0.05, ns, non-significant). Each experiment was repeated two times independent of each other.