FIGURE 1.

RNA-sequencing. (A) Study design. A single trophectoderm (TE) biopsy was analysed by next-generation sequencing (NGS). Blastocysts were classified as i) euploid (EU): <30% aneuploid cells, ii) low-level mosaic (LM): 30%-<50% aneuploid cells, and iii) high-level mosaic (HM): 50%–<70% aneuploid cells. The remaining TE (grey cells) and the inner cell mass (ICM) (black cells) were separately collected from each blastocyst and analysed by RNA sequencing (RNA-seq). (B) Transcriptome sequencing reads of TE and ICM samples. Trimmed reads were obtained through the elimination of adapter sequences and the removal of poor-quality bases from input reads. Mapped reads were aligned to the reference genome.