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. 2023 Apr 11;4(4):101003. doi: 10.1016/j.xcrm.2023.101003

Figure 2.

Figure 2

GT1.2 primes CD4bs-directed VRC01-class serum responses in a gl-CH31 KI mouse model

(A) Schematic of the simple prime-polish immunization regimen. The immunizations are indicated by syringes.

(B) Normalized area under the curve (AUC) values of serum antibody binding to the indicated Env as measured by ELISA, for time point T1 (left) and T4 (right).

(C) Midpoint titers (ID50) of serum against VRC01-class signature viruses as described previously (LaBranche et al.54) for each time point (T1, T4, and T5).

(D) Schematic of the complex sequential immunization regimen.

(E–G) Normalized AUC values of serum antibody binding to the indicated Env as measured by ELISA, for time point T2 (E), T3 (F), or T4 (G). The background color represents the priming group (GT1.2 trimer/NP vs. BG505).

(H) CD4bs specificity of mice primed with GT1.2 (blue, n = 16) or BG505 (gray, n = 8) for each time point, represented by the BG505/BG505 D368R AUC ratio as measured by ELISA.

(I) CD4bs specificity of mice primed with GT1.2 (blue, n = 16) or BG505 (gray, n = 8) at T7 for each of BG505 (clade A), AMC008 (clade B), or ZM197M (clade C) Envs as in (H).

(J) Midpoint neutralization titers (ID50) of serum from mice primed with GT1.2 (blue, n = 16) or BG505 (gray, n = 8) against VRC01-class signature viruses as in (C) for each time point (T2/T6/T7). Each dot represents an individual mouse.