TABLE 1.

Recommended conditions for optimal culture growth of Daphnia as outlined in the OECD test guideline for acute toxicity (OECD 202).

Factor	Optimal range
pH	6–9
Temperature	18–22°C
Dissolved oxygen	>6 mg/L, ideally at saturation
Water Hardness	140–250 mg CaCO3/L
Light/dark cycle	16 light/8 dark