Video 3.
Basal cells stalled in G1 of their cell cycle show low levels of Ca2+ signaling. Disrupted Ca2+ dynamics in G2-depleted Cdkn1b mice (K14-rtTA; tetO-Cdkn1b; R26-GCaMP6s) on the right compared to littermate controls (K14-rtTA; R26-GCaMP6s) on the left, 3 d after start of doxycycline administration. Green fluorescence intensity represents relative cytosolic Ca2+ levels. Frames were taken every 2 s for a total duration of 30 min. Movie shows dynamics at 40 frames per second or 80× the actual timescale. Scale bar: 25 μm.