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. 2023 Apr 27;222(7):e202302095. doi: 10.1083/jcb.202302095

Video 6.

Cx43 orchestrates Ca2+ signaling at large scales, but not across local neighborhoods, in the stem cell pool. Disrupted Ca2+ dynamics in Cx43 cKO mice (K14CreER; Cx43fl/fl; R26-GCaMP6s) on the right compared to littermate controls (K14CreER; Cx43+/+; R26-GCaMP6s) on the left, 5 d after tamoxifen induction. Green fluorescence intensity represents relative cytosolic Ca2+ levels. Frames were taken every 2 s for a total duration of 30 min. Movie shows dynamics at 40 frames per second or 80× the actual timescale. Scale bar: 25 μm.