Figure S2. Myc deficiency in NK cells moderately impacts IL-15 receptor expression.

(A, B) Graphs illustrate a quantification of CD122 and CD132 expressions (geometric mean) on NK cells (NK1.1⁺ CD3/19⁻) of the spleen (A) and BM (B) from mice from the indicated genotypes. (C) Total NK cells were enriched from the spleens of Ncr1cre Myc^fl/fl and Ncr1cre Myc^wt/wt mice and the protein expression levels of STAT5, Erk2, S6, and Rpl11 were assessed by Western blotting. Histone H3 levels are shown as loading controls. (D) DERL-7 cells were pretreated (30 min) or not with Torin2 (250 nM), PD98059 (10 μM), tofacitinib (90 nM), and then stimulated with 25 ng/ml IL-15 for 35 min and the phosphorylation of STAT5, Erk, S6, and p70 S6 kinase was assessed by Western blot. β-Actin levels are shown as loading control. (A, B) Results for CD122 are mean ± SEM of n = 12–13 mice per genotype and are a pool of three independent experiments; the average of Ncr1cre Myc^wt/wt MFI in each experiment was set to 100%. For CD132, results are presented as mean ± SEM of n = 3–4 mice per genotype. (D) Results are representative of two independent experiments (D). Statistical comparisons are shown; **P ≤ 0.01, **P ≤ 0.01, NS, non-significant; t test, unpaired.