TABLE 2.

Primer sequences of target genes.

Genes a	Forward primer (5′-3′)	Reverse primer (5′-3′)	Length	TM b °C	Accession No
OCLN	ACG​GCA​GCA​CCT​ACC​TCA​A	GGG​CGA​AGA​AGC​AGA​TGA​G	123	51.7	XM_025144247.2
CLDN1	CAT​ACT​CCT​GGG​TCT​GGT​TGG​T	GACAGCCA TCCGCA TCTTCT	100	51.3	NM_001013611.2
CLDN2	CCT​ACA​TTG​GTT​CAA​GCA​TCG​TGA	GAT​GTC​GGG​AGG​CAG​GTT​GA	131	50.3	NM_001277622.1
ZO-1	CTT​CAG​GTG​TTT​CTC​TTC​CTC​CTC	CTGTGGTTTCA TGGCTGGATC	144	51.5	XM_021098886.1
TNF-α	GAG​CGT​TGA​CTT​GGC​TGT​C	AAGCAACAACCAGCTA TGCAC	176	55.4	NM_214022.1
IL-1β	ACTGGGCA TCAAGGGCTA	GGTAGAAGA TGAAGCGGGTC	154	55.6	NM_214005.1
IL-6	GCTGCGCTTCTACACAGA	TCCCGTTCTCA TCCA TCTTCTC	203	55.4	NM_204628.1
IL-10	AGA​AAT​CCC​TCC​TCG​CCA​AT	AAA​TAG​CGA​ACG​GCC​CTC​A	121	51.2	NM_001004414.2
GAPDH	TGC​TGC​CCA​GAA​CAT​CAT​CC	ACG​GCA​GGT​CAG​GTC​AAC​AA	142	50-60	NM_204305

^a Primer sequences of OCLN, CLDN1, CLDN2, ZO-1, TNF-α, IL-1β, IL-6, IL-10, and GAPDH.

^b TM, melting temperature.