REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-Cas9	Cell Signaling Technology	Cat# 14697; RRID:AB_2750916
Rabbit monoclonal anti-AR	Cell Signaling Technology	5153Cat# 5153; RRID:AB_10691711
Rabbit monoclonal anti-β-Actin	Cell Signaling Technology	Cat#4970; RRID:AB_2223172
Rabbit polyclonal anti-CK1α	Cell Signaling Technology	Cat# 2655; RRID:AB_2283593
Rabbit monoclonal anti-ATM	Cell Signaling Technology	Cat# 2873; RRID:AB_2062659
Rabbit polyclonal anti-CHK2	Cell Signaling Technology	Cat# 2662; RRID:AB_2080793
Rabbit monoclonal anti-phospho-CHK2 (T68)	Cell Signaling Technology	Cat# 2197; RRID:AB_2080501
Rabbit monoclonal anti-γH2AX	Cell Signaling Technology	Cat# 9718; RRID:AB_2118009
Rabbit monoclonal anti-H2AX	Cell Signaling Technology	Cat# 7631, RRID:AB_10860771
Rabbit monoclonal anti-DNA-PKcs	Cell Signaling Technology	Cat# 38168; RRID:AB_2799128
Rabbit monoclonal anti-Cleaved PARP	Cell Signaling Technology	Cat# 5625; RRID:AB_10699459
Rabbit monoclonal anti-Myc-Tag	Cell Signaling Technology	Cat# 2278, RRID:AB_490778
Rabbit monoclonal anti-HA-tag	Cell Signaling Technology	Cat# 3724; RRID:AB_1549585
Rabbit polyclonal anti-Cleaved Caspase-3	Cell Signaling Technology	Cat# 9661, RRID:AB_2341188
Goat polyclonal anti-rabbit IgG, HRP-linked	Cell Signaling Technology	Cat# 7074; RRID:AB_2099233
Goat polyclonal anti-mouse IgG, HRP-linked	Cell Signaling Technology	Cat# 7076; RRID:AB_330924
Mouse monoclonal anti-alpha-Tubulin	Cell Signaling Technology	Cat# 3873; RRID:AB_1904178
Mouse monoclonal anti-vinculin	Sigma-Aldrich	Cat# V4505; RRID:AB_477617
Rabbit polyclonal anti-FLAG	Sigma-Aldrich	Cat# F7425; RRID:AB_439687
Mouse monoclonal anti-CK1α	Santa Cruz Biotechnology	Cat# sc-74582; RRID:AB_2084662
Mouse monoclonal anti-p53	Santa Cruz Biotechnology	Cat# sc-126; RRID:AB_628082
Mouse monoclonal anti-HA Magnetic Beads	Thermo Fisher Scientific	Cat# 88836; RRID:AB_2749815
Mouse monoclonal anti-FLAG Magnetic Beads	Sigma-Aldrich	Cat# M8823; RRID:AB_2637089
Rabbit monoclonal anti-ATM	Abcam	Cat# ab201022
Rabbit monoclonal anti-ATM	Abcam	Cat# ab32420; RRID:AB_725574
Rabbit monoclonal anti-CK1α	Abcam	Cat# ab223144
Rabbit polyclonal anti-CK1α	OriGene	Cat# TA313698
Rabbit polyclonal anti-phospho-ATM (S1270)	This paper	N/A
Goat polyclonal anti-Rabbit IgG	Thermo Fisher Scientific	Cat# A-11034; RRID:AB_2576217
Bacterial and virus strains
lentiCas9-Blast	Sanjana et al.42	Addgene Cat# 52962
lentiGuide-Puro	Sanjana et al.42	Addgene Cat# 52963
LentiGuidPuro-hTP53_sgRNA-1	Wang et al.43	Addgene Cat# 88853
sgTP53_3	Hong et al.44	Addgene Cat# 78164
pcDNA3.1(+) FLAG-His-ATM wt	Canman et al.45	Addgene Cat# 31985
PRKDC gRNA1	Doench et al.46	Addgene Cat# 77861
PRKDC gRNA2	Doench et al.46	Addgene Cat# 77862
ATM gRNA1	Doench et al.46	Addgene Cat# 77530
ATM gRNA2	Doench et al.46	Addgene Cat# 77531
CHEK2 gRNA1	Doench et al.46	Addgene Cat# 76487
CHEK2 gRNA2	Doench et al.46	Addgene Cat# 76488
CSNK1A1 gRNA1	Doench et al.46	Addgene Cat# 76188
CSNK1A1 gRNA2	Doench et al.46	Addgene Cat# 76189
Tet-plko-puro	Wiederschain et al.47	Addgene Cat# 21915
pLC-FLAG-CSNK1A1-WT-Puro	Patil et al.48	Addgene Cat# 123319
pcDNA3.1-HA	Oskar Laur	Addgene Cat# 128034
psPAX2	Didier Trono	Addgene Cat# 12260
pCMV-VSV-G	Stewart et al.49	Addgene Cat# 8454
GST-ATM fragments	Dr. Zhenkun Lou Lab (Mayo Clinic, Rochester, USA)	N/A
Endura Competent Cells	Lucigens	Cat# 60242
Biological samples
Patient-derived xenografts (PDX)	University of Washington	LuCaP 35CR
Patient-derived xenografts (PDX)	University of Washington	LuCaP 77CR
Chemicals, peptides, and recombinant proteins
Enzalutamide	Selleck Chemicals	Cat# S1250
doxycycline	Selleck Chemicals	Cat# S5159
cycloheximide	Selleck Chemicals	Cat# S7418
MG132	Selleck Chemicals	Cat# S2619
D4476	Selleck Chemicals	Cat# S7642
BTX-A51	DC Chemicals	Cat# DC11250
CK1α	Thermo Fisher Scientific	Cat# PV3850
ATM	Sigma-Aldrich	Cat# 14-933
Polybrene	Sigma-Aldrich	Cat# TR-1003-G
Hoechst	Sigma-Aldrich	Cat# B2261
Kinase Buffer	Cell Signaling Technology	Cat# 9802
Lipofectamine 2000	Thermo Fisher Scientific	Cat# 11668019
DMSO	Sigma-Aldrich	Cat# D2650
carboxymethyl cellulose	Selleck Chemicals	Cat# S6703
Tween 80	Sigma-Aldrich	Cat# P4780
Solutol	MedChemExpress	Cat# HY-Y1893
2-hydroxy prolyl-b-cyclodextrin	MedChemExpress	Cat# HY-101103
AquaBluer Solution	MultiTarget	Cat# 6015
Critical commercial assays
Q5 Site-Directed Mutagenesis Kit	New England Biolabs	Cat# E0554
RNeasy Mini kit	Qiagene	Cat# 74104
Plasmid Maxi Kit	Qiagene	Cat# 12162
Deposited data
Raw and analyzed data	This paper	GSE203362
Experimental models: Cell lines
22Rv1	ATCC	CRL-2505
C4-2	ATCC	CRL-3314
LNCaP	ATCC	CRL-1740
MR49F	Dr. Amina Zoubeidi Lab (University of British Columbia, Vancouver, BC, Canada)	N/A
C4-2R	Dr. Allen Gao Lab (University of California, Davis, CA, USA)	N/A
C4-2B	Dr. Ka Wing Fong (University of Kentucky, USA)	N/A
HEK293T	Dr. Andrea Kasinski Lab (Purdue University, USA)	N/A
Experimental models: Organisms/strains
NU/J mice	The Jackson Laboratory	RRID:IMSR_JAX:002019
NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ mice	The Jackson Laboratory	RRID:IMSR_JAX:005557
Oligonucleotides
sgAR-full#1 5′GTTACACGTGGACGACCAGA-3′	This paper	N/A
sgAR-full#2 5′GTGTCCAGCACACACTACACC-3′	This paper	N/A
shCK1α#1 5′GCCACAGTTGTGATGGTTGTT-3′	This paper	N/A
shCK1α#2 5′GCAAGCTCTATAAGATTCTTC-3′	This paper	N/A
Recombinant DNA
Myc-DDK-CSNK1A1 (#RC217936)	OriGene	Cat# RC217936
PcDNA3.1-HA-CK1α	This paper	N/A
Tet-plko-puro-CK1α	This paper	N/A
sgAR-full length	This paper	N/A
Kinome CRISPR Knockout Library	Doench et al.46	Addgene Cat# 75314
Software and algorithms
MAGeCK	Li et al.50	https://sourceforge.net/p/mageck/wiki/Home/
R package limma	Ritchie et al.51	https://bioconductor.org/packages/release/bioc/html/limma.html
GSEA4.1.0	Subramanian et al.52	https://www.gsea-msigdb.org/gsea/index.jsp
ImageJ	Schneider et al.53	https://imagej.nih.gov/ij/
GraphPad Prism 8.0	GraphPad	https://www.graphpad.com/