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. 2023 Apr 18;27:327–336. doi: 10.1016/j.bioactmat.2023.04.001

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© 2023 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Cellular composition of early-stage atherosclerotic plaque one month after treatment with a single dose of miR-145 micelles. (A) Immunohistochemistry of aortic root plaques for eYFP (green), α-SMA, CD68, RUNX2 (red), and nuclei (blue). Red boxes on H&E images indicate zoomed in areas of aortic root (bottom). Quantification of cells expressing (B) α-SMA+/DAPI, (C) α-SMA + YFP+/YFP+, (D) CD68+/DAPI, (E) CD68 + YFP+/YFP+, (F) RUNX2+/DAPI, and (G) RUNX2+YFP+/YFP+. VSMC marker expression with respect to YFP is used to analyze all VSMC-originating cells. * indicates p < 0.05, ** indicates p < 0.01.