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. 2023 Feb 16;15(6):1325–1350. doi: 10.1016/j.jcmgh.2023.02.004

Figure 11.

Figure 11

Stable overexpression of wild-type (WTO but not the phosphatase defective mutant form of PFKFB4 could rescue the PFKFB4 KO–induced suppression of subcutaneous tumor growth in vivo. (A) Western blot analysis for PFKFB4 protein expression in vector-rescued control KO cells and vector, wild-type PFKFB4, and phosphatase defective PFKFB4-rescued PFKFB4 KO Huh7 cells subjected to normoxic and hypoxic conditions for 24 hours. (B) Comparison of the growth rates among the vector-rescued control KO cells and vector, wild-type PFKFB4, and phosphatase defective PFKFB4-rescued PFKFB4 KO cell–derived subcutaneous tumor xenografts. (C) The tumor volume of the dissected tumors among different experimental groups was compared at the end point. (D) The tumor mass and tumor volume of the control, PFKFB4 WT, and PFKFB4 H257A mutant stable overexpressing Huh7 cell–derived subcutaneous tumor xenografts after dissection. ∗P < .05. sgGFP, single-guide RNA against Green Fluorescent Protein.