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Primers for Cloning PFKFB4 Overexpression Construct
| Primer | Sequence, 5’>3’ |
|---|---|
| PFKFB4 AgeI F | GGACCGGTGCCACCATGGCGTCCCCACGGGAATT |
| PFKFB4 480 BamHI R | CAGGATCCACACAGATGGAC |
| PFKFB4 480 BamHI F | GTGGATCCTGAGGTCATAGC |
| PFKFB4 stop-1 BamHI R | CCGGATCCCTGGTGAGCAGGCACCGTGA |
| PFKFB4 H257A F | CTCTGCCGGGCCGGGGAGAGC |
| PFKFB4 H257A R | GCTCTCCCCGGCCCGGCAGAG |
F, forward primer; R, reverse primer.
