Table 1.
Species | Primer | Sequence 5’ to 3’ |
---|---|---|
Plasmodium spp. | FIP | TACGGCCCGACGGTAAGATCGTAACCATGCCAACAC |
BIP | AGGAGTCTCACACTAGCGACAAAATTCCTTGTCGGGTAATCTC | |
FLP | Biotin-CCGTCATAGCCATGTTAG | |
BLP | DIG-ACCACATCTAAGGAAGGCAG | |
F3 | TGTCAACTACCATGTTACGAC | |
B3 | AACGGTCCTAAGGTAGCAA | |
P. knowlesi | FIP | GTTGTTGCCTTAAACTTCCTTGTGTTCTTGATTGTAAAGCTTCTTAGAGG |
BIP | TGATGTCCTTAGATGAACTAGGCTTTGCAAGCAGCTAAAATCGT | |
FLP | Biotin-TAGACACACATCGTT | |
BLP | FAM-GCACGCGTGCTACACT | |
F3 | CCATCTATTTCTTTTTTGCGTATG | |
B3 | CAGTGGAGGAAAAGTACGAA | |
P. vivax | FIP | GCCATGTTAGGCCAATACCCTAATGTGTGTATCAATCGAGTTTCT |
BIP | TAACGGGGAATTAGAGTTCGATTCCTGTAATTTACGCGCCTGCT | |
FLP | Biotin-CATCAAAAGCTGATAGGTC | |
BLP | DNP-GGAGAGGGAGCCTGAGAAATAGC | |
F3 | AGCGACACGTAATGGATC | |
B3 | CTTGTCACTACCTCTCTTCT | |
P. falciparum | FIP | AGTAGTCCGTCTCCAGAAAATCTTACTTTGGGGGCATTCGTATT |
BIP | GCGAAAGCATTTGCCTAATCTATTTAAGATTACGACGGTATCTGATC | |
FLP | Biotin-TCACCTCTGACATCTG | |
BLP | Cy5- GTTAAGGGAGTGAAGACG | |
F3 | GCTTAGTTACGATTAATAGGAGTA | |
B3 | AGTCGGCATAGTTTATGGT |
FIP: Forward inner primer; BIP: backward inner primer; FLP: forward loop primer; BLP: backward loop primer; F3: forward primer; B3: backward primer.