Table 3.
Samples | Microscopy | Nested PCR | Multiplex LAMP-LF | |
---|---|---|---|---|
P. knowlesi | Positive | 31 + β1 | #1 + *1 + 31 | *1 + β1 + 31 |
Negative | 0 | 0 | 0 | |
P. falciparum | Positive | *1 + 2 | 2 | 2 |
Negative | 0 | 0 | 0 | |
P. vivax | Positive | 7 | α1 + 7 | 7 |
Negative | 0 | 0 | 0 | |
Negative | Positive | 0 | 0 | 0 |
Negative | #1 + α1 + 42 | 42 + β1 | #1 + α1 + 42 |
# Sample was detected negative by microscopy and multiplex LAMP-LF but as P. knowlesi by nested PCR; α Sample was detected negative by microscopy and multiplex LAMP-LF but as P. vivax by nested PCR; β Sample was detected as P. knowlesi by microscopy and multiplex LAMP-LF but negative by nested PCR; * Sample was detected as P. falciparum by microscopy and P. knowlesi by multiplex LAMP-LF and nested PCR.