. 2023 Mar 29;11(4):873. doi: 10.3390/microorganisms11040873

Table 1.

Antimicrobial reagent concentrations on Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Enterobacter aerogenes, Serratia marcescens, Candida tropicalis, Lodderromyces elongisporus, Aspergillus aculeatus and Penicillium citrinum during contact time of 10 min.

Reagents	Concentration (%)	Inhibitory Effect (Log Reduction ± SD)
		(+ve) Bacteria				(−ve) Bacteria		Yeast		Mold
		B. cereus	B. subtilis	S. aureus	S. epidermidis	E. aerogenes	S. marcescens	C. tropicalis	L. elongisporus	A. aculeatus	P. citrinum
KMS	1	1.30 ± 0.25 ^Cc	1.97 ± 0.26 ^Ba	0.53 ± 0.41 ^Da	1.27 ± 0.09 ^Cc	0.45 ± 0.14 ^Deb	0.47 ± 0.14 ^DEabc	0.34 ± 0.19 ^DEc	0.66 ± 0.87 ^Da	6.30 ± 0.35 ^Aa	- ^{* Eb}
	2.5	1.65 ± 0.32 ^Cb	2.04 ± 0.02 ^Ca	0.18 ± 0.14 ^Db	1.86 ± 0.68 ^Cc	0.68 ± 0.09 ^Da	0.53 ± 0.23 ^Dab	0.52 ± 0.13 ^Dc	0.93 ± 0.92 ^Da	6.30 ± 0.35 ^Ba	6.44 ± 0.16 ^Aa
	3	1.74 ± 0.19 ^Cb	1.93 ± 0.22 ^Cb	0.32 ± 0.12 ^Eab	1.62 ± 0.05 ^Cbc	0.60 ± 0.02 ^DEa	0.34 ± 0.13 ^Ec	0.80 ± 0.35 ^DEc	0.94 ± 0.90 ^Da	6.30 ± 0.35 ^Ba	6.44 ± 0.16 ^Aa
	5	1.74 ± 0.23 ^Db	1.73 ± 0.06 ^Dab	0.24 ± 0.13 ^Fb	3.77 ± 0.30 ^Ca	0.48 ± 0.10 ^Fb	0.37 ± 0.01 ^Fbc	3.80 ± 0.46 ^Cb	1.21 ± 0.82 ^Ea	6.30 ± 0.35 ^Ba	6.44 ± 0.16 ^Aa
	10	3.04 ± 0.19 ^Ea	1.89 ± 0.00 ^Fab	0.34 ± 0.06 ^Gab	4.01 ± 0.04 ^Da	0.67 ± 0.00 ^Ga	0.64 ± 0.08 ^Ga	5.37 ± 0.28 ^Cb	1.92 ± 0.88 ^Fa	6.30 ± 0.35 ^Ba	6.44 ± 0.16 ^Aa
NaOCl	0.0005	2.65 ± 0.32 ^Eb	2.78 ± 0.21 ^Ea	7.82 ± 0.09 ^Ba	4.98 ± 0.14 ^Dc	7.55 ± 0.07 ^Bb	8.33 ± 0.05 ^Aa	6.44 ± 0.16 ^Ca	6.44 ± 0.88 ^Ca	1.78 ± 0.26 ^Fb	- ^Gc
	0.0010	2.44 ± 0.09 ^Db	1.88 ± 0.20 ^Eb	7.82 ± 0.09 ^Ba	6.81 ± 0.32 ^Cb	7.63 ± 0.11 ^Bb	8.33 ± 0.05 ^Aa	6.44 ± 0.16 ^Ca	6.44 ± 0.88 ^Ca	1.26 ± 0.08 ^Fc	0.22 ± 0.12 ^Gc
	0.0015	2.65 ± 0.07 ^Db	1.71 ± 0.12 ^Eb	7.82 ± 0.09 ^Ba	8.31 ± 0.04 ^Aa	8.43 ± 0.03 ^Aa	8.33 ± 0.05 ^Aa	6.44 ± 0.16 ^Ca	6.44 ± 0.88 ^Ca	2.08 ± 0.24 ^Eab	0.96 ± 0.33 ^Fb
	0.0020	3.04 ± 0.19 ^Da	1.75 ± 0.02 ^Fb	7.82 ± 0.09 ^Ba	8.31 ± 0.04 ^Aa	8.43 ± 0.03 ^Aa	8.33 ± 0.05 ^Aa	6.44 ± 0.16 ^Ca	6.44 ± 0.88 ^Ca	2.26 ± 0.28 ^Ea	1.92 ± 0.38 ^EFa
NaCl	5	0.85 ± 0.09 ^Bc	- * ^Cc	- ^Ca	0.01 ± 0.11 ^Cb	0.65 ± 0.01 ^Bb	1.46 ± 0.09 ^Ab	0.24 ± 0.20 ^Cb	0.56 ± 0.94 ^Bb	- ^Ca	- ^Ca
	10	1.20 ± 0.14 ^Aab	0.54 ± 0.40 ^Bb	- ^Ca	0.25 ± 0.12 ^BCab	0.55 ± 0.01 ^Bc	1.39 ± 0.11 ^Ab	0.37 ± 0.14 ^BCab	0.62 ± 0.87 ^Ba	- ^Ca	- ^Ca
	15	1.23 ± 0.15 ^Aab	- ^Dc	- ^Da	0.19 ± 0.03 ^CDab	0.49 ± 0.05 ^BCd	1.41 ± 0.04 ^Ab	0.15 ± 0.03 ^CDb	0.55 ± 0.94 ^Bb	- ^Da	- ^Da
	20	1.45 ± 0.35 ^ABa	1.08 ± 0.5 ^Ca	- ^Da	0.25 ± 0.02 ^Dab	0.73 ± 0.07 ^Ca	1.63 ± 0.11 ^Aa	0.26 ± 0.23 ^Db	0.64 ± 0.89 ^Cb	- ^Da	- ^Da
	30	1.02 ± 0.22 ^Bbc	0.58 ± 0.43 ^BCDb	- ^Fa	0.26 ± 0.31 ^DEFa	0.51 ± 0.03 ^CDEcd	1.49 ± 0.16 ^Ab	0.76 ± 0.50 ^BCa	0.46 ± 0.83 ^BCDb	- ^Fa	- ^Fa
Citric acid	5	1.51 ± 0.08 ^Ea	2.30 ± 0.00 ^Db	0.98 ± 0.05 ^Fe	8.33 ± 0.00 ^Aa	6.14 ± 0.13 ^Bd	5.24 ± 0.18 ^Cc	0.33 ± 0.21 ^GHb	0.50 ± 0.88 ^Gb	- ^Ha	- ^Ha
	10	1.34 ± 0.08 ^Fa	2.34 ± 0.00 ^Db	1.60 ± 0.34 ^Ed	8.33 ± 0.00 ^Aa	6.54 ± 0.13 ^Bc	5.47 ± 0.01 ^Cb	0.88 ± 0.70 ^Gb	0.52 ± 0.86 ^Gb	- ^Ha	- ^Ha
	15	1.47 ± 0.10 ^Da	2.46 ± 0.06 ^Ca	2.73 ± 0.21 ^Cc	8.33 ± 0.00 ^Aa	6.65 ± 0.03 ^Bb	8.33 ± 0.05 ^Aa	0.92 ± 0.54 ^Db	0.52 ± 0.91 ^Db	- ^Fa	- ^Fa
	20	1.21 ± 0.08 ^CDa	2.56 ± 0.13 ^Ca	4.12 ± 0.16 ^Bb	8.33 ± 0.00 ^Aa	8.43 ± 0.03 ^Aa	8.33 ± 0.05 ^Aa	0.65 ± 0.72 ^Db	1.01 ± 0.98 ^Db	- ^Ea	- ^Ea
	30	1.50 ± 0.20 ^EFa	2.56 ± 0.15 ^Da	7.82 ± 0.09 ^Ba	8.33 ± 0.00 ^Aa	8.43 ± 0.03 ^Aa	8.33 ± 0.05 ^Aa	2.31 ± 0.11 ^Ea	3.67 ± 0.75 ^Ca	- ^Fa	- ^Fa
Salt/acid	15:10	0.86 ± 0.13 ^Ea	3.50 ± 0.13 ^Ca	4.22 ± 0.25 ^Bb	8.31 ± 0.00 ^Aa	8.43 ± 0.00 ^Aa	8.34 ± 0.00 ^Aa	3.07 ± 0.23 ^Db	0.45 ± 0.22 ^Fc	- ^Fa	- ^Ga
(NaCl: citric acid)	15:15	0.94 ± 0.37 ^Da	3.30 ± 0.11 ^Cab	4.80 ± 0.30 ^Bb	8.31 ± 0.00 ^Aa	8.43 ± 0.00 ^Aa	8.34 ± 0.00 ^Aa	3.43 ± 0.34 ^Cb	0.66 ± 0.44 ^Dc	- ^Ea	- ^Ea
	15:20	1.12 ± 0.13 ^Fa	2.95 ± 0.45 ^Dbc	6.67 ± 1.35 ^Ba	8.31 ± 0.00 ^Aa	8.43 ± 0.00 ^Aa	8.34 ± 0.00 ^Aa	4.19 ± 0.44 ^Ca	1.86 ± 0.20 ^Eb	- ^Ga	- ^Ga
	15:30	0.91 ± 0.13 ^Ea	2.69 ± 0.35 ^Dc	7.03 ± 0.92 ^Ba	8.31 ± 0.00 ^Aa	8.43 ± 0.00 ^Aa	8.34 ± 0.00 ^Aa	4.67 ± 0.31 ^Ca	2.79 ± 0.17 ^Da	- ^Fa	- ^Fa
Range of inhibitory effect			0–1.00	1.01–2.00	2.01–3.00	3.01–4.00	4.01–5.00	5.01–6.00	6.01–7.00	7.01–8.00	8.01–9.00

Values are average mean ± standard deviation of two replicates. Different superscript small letters within a column indicate significant differences between concentration at the level of p < 0.05. Different superscript capital letters within a row indicate significant differences between microbial species at the level of p < 0.05. Note: * = No inhibitory effect. Initial load of B. cereus, B. subtilis, S. aureus, S. epidermidis, E. aerogenes, S. marcescens, C. tropicalis, L. elongisporus, A. aculeatus and P. citrinum were 7.0, 7.2, 7.8, 8.3, 8.4, 8.3, 6.4, 6.4, 6.3 and 6.4 log CFU/mL, respectively.