Table 1.

Optimum method parameters determined for the flow-cytometry-based quantification of BVs after the infection of Sf9 cells in a 96-well format. The procedure without staining is only suitable for viruses expressing a fluorophore (e.g., GFP), whereas the staining procedure targets the gp64 surface protein, which is present on all BVs, independent of the genotype.

Parameter [Unit]	No Staining	PE Staining	APC Staining
Seeding cell concentration [cells/mL]	0.8 × 106	1.0 × 106	1.0 × 106
Volume per well [µL]	150	150	150
Incubation time of virus infection [h]	17	5	4
Concentration of fluorescent antibody [mg/mL]	Not applicable	0.001	0.00025
Volume per well [µL]		50	50
Incubation time of antibody staining [min]	Not applicable	30	15 *

* The incubation can be performed at 4 °C or at room temperature, without affecting the results.