TABLE 2.
Physical and biological parameters for the samples from which the libraries were generated
| Marine region | Depth (m) | Temp (°C) | Salinity (‰) | Chlorophyll concn (μg liter−1) | Prokaryote concn (105 cells ml−1) | DNA yield (μg liter−1) | Library(ies)a |
|---|---|---|---|---|---|---|---|
| North Atlantic Ocean | 5 | 11.8 | 35.33 | 0.85 | 4.18 | 1.30 | AT-5 |
| 200 | 8.8 | 35.29 | 0.04 | 1.16 | 0.33 | AT-200 | |
| Cantabrian Sea | 15 | 14.3 | 35.67 | 0.50 | 5.60 | 0.69 | CA-15 |
| Alboran Sea | 5 | 18.1 | 36.56 | 0.98 | 6.35 | 0.73 | ME-5 |
| 450 | 13.2 | 38.50 | 1.46 | 0.11 | ME-450 | ||
| Santa Barbara Channel | 0 | 13.9 | 33.41 | SB-0 | |||
| 200 | 9.0 | 34.05 | SB-200, SB-200B | ||||
| Drake Passage, north | 5 | 6.9 | 34.12 | 0.35 | 3.34 | 0.31 | DN-5 |
| 200 | 4.9 | 34.21 | 0.04 | 1.41 | 0.19 | DN-200 | |
| Drake Passage, frontal zone | 5 | 7.2 | 34.10 | 0.45 | 5.46 | 0.33 | DF-5 |
| 200 | 4.9 | 34.20 | 0.06 | 1.33 | 0.12 | DF-200 | |
| Drake Passage, south | 5 | 0.7 | 34.14 | 0.43 | 2.01 | 0.27 | DS-5 |
| 200 | 0.2 | 34.37 | 0.05 | 3.13 | 0.09 | DS-200 | |
| Arthur Harbor (4 August 1996)b | 20 | −1.8 | 33.70 | 0.10 | 1.50 | AM-20A | |
| Arthur Harbor (16 August 1996) | 20 | AM-20B |
a
Most libraries were derived from amplicons produced with primers 21f and 958r. The exceptions were the SB-200B library (derived from amplicons produced with primers 20f and 1392r) and the AM-20A and AM-20B libraries (derived from amplicons produced with primers 20f and 958r).
b
Average data for September 1996.