Table 1.
Reference values for the various assays obtained in WT ABCA3 transfected cells without treatment.
| Code | Name of Assay | Absolute Results (Mean ± SD; n) | Mean Value Set to 1 | Normalized SD (nSD; SD Expressed as a Fraction of the Mean Value Set to 1 in Each Experiment) | ||
|---|---|---|---|---|---|---|
| Mean | Median | Range | ||||
| A | % ER localization of ABCA3 | 33.33 ± 23.63%; 1 ** | 1 | 0.71 | 0.71 | - |
| B | N-glycosylation of ABCA3 * | 63.12 ± 10.62%, 8 | 1 | 0.17 | 0.18 | 0.11–0.21 |
| C | % Lysosomal localization of ABCA3 | 95.00 ± 8.66%; 1 ** | 1 | 0.09 | 0.09 | - |
| D | Proteolytic cleavage of ABCA3 | 0.29 ± 0.10; 12 | 1 | 0.31 | 0.32 | 0.18–0.48 |
| E | Volume of ABCA3+ vesicles | 1.06 ± 0.17 µm3; 9 | 1 | 0.16 | 0.12 | 0.07–0.28 |
| F | Amount of PC from recycling into ABCA3+ vesicles * | 25,000 ± 4330 AU; 6 | 1 | 0.13 | 0.13 | 0.09–0.17 |
| G | Amount of PC from de novo synthesis into ABCA3+ vesicles | 65,149 ± 11,312 AU; 9 | 1 | 0.18 | 0.17 | 0.14–0.23 |
| H | ATPase activity of ABCA3 * | 100 ± 14.35%; 24 | 1 | 0.14 | 0.13 | 0.12–0.19 |
The mean values and standard deviation (SD) values were collected from experiments with wild-type cells in this study. * The mean values and corresponding SD values were collected as approximate values from graphs in the literatures due to the lack of exact values. If there was only an SEM approximate value, then the SD value was calculated manually as the SEM value multiplied by the square root of n (i.e., number of independent experiments). For further definition, the mean value of the SD was selected. ** Sixty randomly selected vesicles from three different fields.