TABLE 2.
Summary of body-fat associated metabolites in the validation cohorts.
| Metabolite | Class | Ion | m/z | RT/RIa | Molecular | Trait | ρ | ρ p-value | ρ′ | ρ' p-value |
|---|---|---|---|---|---|---|---|---|---|---|
| Validation 1: Caucasian women (n = 136) | ||||||||||
| Acetylglycine | Amino acids | Pos | 140.03 | 1.17 | C4H7NO3 | AG | −0.31 | 1.96 × 10−4 | −0.28 | 9.10 × 10−4 |
| AW | −0.29 | 6.89 × 10−4 | −0.25 | 3.27 × 10−3 | ||||||
| GW | 0.29 | 5.88 × 10−4 | 0.28 | 1.17 × 10−3 | ||||||
| W% | −0.34 | 5.17 × 10−5 | −0.29 | 6.55 × 10−4 | ||||||
| Creatinine | Amino acids | Neg | 112.05 | 0.98 | C4H7N3O | AW | −0.20 | 1.96 × 10−2 | −0.15 | 7.61 × 10−2 |
| Thiamine | Pyrimidines | Pos | 265.11 | 1.13 | C12H16N4OS | W% | −0.19 | 2.34 × 10−2 | −0.16 | 7.01 × 10−2 |
| Validation 2: African American and Caucasian men (n = 700) | ||||||||||
| Acetylglycine | Amino acids | Polar | 116.04 | 1,780 | C4H7NO3 | AG | −0.26 | 7.22 × 10−12 | −0.24 | 1.47 × 10−10 |
| AW | −0.28 | 6.48 × 10−14 | −0.26 | 1.97 × 10−12 | ||||||
| GW | 0.09 | 1.41 × 10−2 | 0.09 | 1.32 × 10−2 | ||||||
| W% | −0.27 | 6.34 × 10−13 | −0.24 | 5.28 × 10−11 | ||||||
| Cortisone | Lipid | Neg | 359.19 | 4,575 | C21H28O5 | AG | −0.11 | 2.67 × 10−3 | −0.10 | 1.12 × 10−2 |
| AW | −0.11 | 2.38 × 10−3 | −0.10 | 8.97 × 10−2 | ||||||
| W% | −0.11 | 5.02 × 10−3 | −0.09 | 1.22 × 10−2 | ||||||
| Creatinine | Amino acids | Pos Early | 114.07 | 2,055 | C4H7N3O | AG | −0.09 | 2.20 × 10−2 | −0.05 | ns |
| AW | −0.09 | 1.84 × 10−2 | −0.03 | ns | ||||||
| W% | −0.16 | 1.24 × 10−5 | −0.13 | 6.69 × 10−4 | ||||||
| Glycocholic acid | Lipid | Neg | 464.30 | 5,163 | C26H43NO6 | AG | −0.10 | 8.16 × 10−3 | −0.07 | ns |
| AW | −0.13 | 5.96 × 10−4 | −0.10 | 8.13 × 10−3 | ||||||
| W% | −0.12 | 1.96 × 10−3 | −0.09 | 1.38 × 10−2 | ||||||
| N-Phenylacetyl-L-Glutamine | Peptide | Pos Early | 265.12 | 2,145 | C13H16N2O4 | AG | −0.02 | ns | −0.11 | 4.17 × 10−3 |
| AW | −0.04 | ns | −0.12 | 1.34 × 10−3 | ||||||
| W% | −0.08 | 2.81 × 10−2 | −0.14 | 2.60 × 10−4 | ||||||
m/z: mass-to-chare ratio; a: RT (Retention time) and RI (Retention index); ρ: Spearman correlation coefficient; ρ': Partial Spearman correlation coefficient adjusted by age, height, and physical activity in Validation 1 and additional ethnicity in Validation 2; ns: not statistically significant; The directions of the correlation test in the validation studies are consistent with the findings in the Chinese women cohort. A/G: android to gynoid ratio; A/W: android fat to whole body total fat; G/W: gynoid fat to whole body total fat; W%: whole body total fat percentage.