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. Author manuscript; available in PMC: 2023 Apr 28.
Published in final edited form as: Gene Ther. 2016 Jul 1;23(10):734–742. doi: 10.1038/gt.2016.52

Figure 1.

Figure 1.

Analysis of alveolar epithelial cell phenotypes in culture. Primary rat alveolar epithelial type II cells were isolated and cultured on glass coverslips alone or coverslips precoated with Matrigel (day 2 only) for either 2, 3, 5 or 7 days. Immunofluorescence was then performed using antibodies specific to the ATII markers, SP-B and LB180, as well as the ATI markers T1α and AQP-5. The rat cell line, R3/1, was also analyzed. Staining for the fibroblast marker, vimentin, was used to verify purity of the cultures.