Fig. 1.

IL-10 expression in mouse tissue leukocytes A VertX mice, in which an IRES-enhanced green fluorescent protein (eGFP) fusion protein was placed downstream of exon 5 of the IL-10 gene, were crossed into the atherosclerosis-prone Apoe^−/− background. B Aortic leukocyte composition as % of live CD45⁺ cells in aortas of VertX Apoe^−/− mice fed WD, (8 weeks, n = 6) or CD (16 weeks, n = 4). C IL-10^GFP+ expression reported as % of parent cell type from aortas of VertX Apoe^−/− mice on CD (16 weeks). D IL-10^GFP+ expression reported as % of parent cell type from aortas of VertX Apoe^−/− mice on WD (8 weeks). E Spleen leukocyte composition expressed as % of total CD45⁺ live cells from VertX Apoe^−/− mice on WD (8 weeks, n = 6) or kept on CD (16 weeks, n = 4). F IL-10^GFP+ expression reported as % of parent cell type of IL-10^GFP+ from spleen of Vert-X Apoe^−/− mice on CD. G IL-10^GFP+ expression reported as % of parent cell type from spleen of VertX Apoe^−/− mice on WD. H Leukocyte composition expressed as % of total CD45⁺, Live cells in peritoneal lavage fluid of Vert-X Apoe^−/− mice challenged with a WD (8 weeks, n = 5) or kept in CD (16 weeks, n = 5). I) IL-10^GFP+ expression reported as % of parent cell type from peritoneal lavage fluid of VertX Apoe^−/− mice on CD. J IL-10^GFP+ expression reported as % of parent cell type from peritoneal lavage fluid of VertX Apoe^−/− mice on WD. K Leukocyte composition expressed as % of total CD45 ⁺ , live cells in draining LNs of Vert-X Apoe^−/− mice challenged with a WD (8 weeks, n = 5) or kept on CD. L IL-10^GFP+ expression reported as % of parent cell type from LNs of VertX Apoe^−/− mice on CD. M IL-10^GFP+ expression reported as % of parent cell type of IL-10^GFP+ from LNs of VertX Apoe^−/− mice on WD. Representative gating scheme in S.Fig. 1. Data are presented as mean ± SEM. P value was calculated by one-way ANNOVA test with Tukey’s correction for multiple comparison *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001