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. 2023 Apr 14;14:1149874. doi: 10.3389/fimmu.2023.1149874

Figure 5.

Figure 5

Histopathological examination of injection sites in a mouse model of IC-induced subcutaneous inflammation. The skin biopsy specimens collected from injected areas of PBS (A–C), TNFα (D–F), ozoralizumab-TNFα IC1 (G–I), ozoralizumab-TNFα IC2 (J–L), and adalimumab-TNFα ICs (M–O) were examined histopathologically after hematoxylin and eosin staining. The center column and right column are high magnification views of the blue boxes and red boxes, respectively, in the left column. The arrows point to focal neutrophil infiltration in the deeper layer of the subcutaneous tissue in the TNFα (D) and adalimumab-TNFα IC (M) injected areas. Neutrophils had also sporadically infiltrated the superficial layer of subcutaneous tissue (arrowheads) in the TNFα (E) and adalimumab-TNFα IC (N) injected areas. By contrast, the areas injected with ozoralizumab-TNFα IC1 (G–I), ozoralizumab-TNFα IC2 (J–L), and PBS (A–C) did not show any evidence of inflammatory cell infiltration. Scale bars: 200 µm (left), 50 µm (center and right).