3D samples segmentation graphical workflow: A) the first step consists in creating a mask of the whole heart. Pixels’ values in the [−200; 1600] HU range are included in the mask. Simultaneously a mask of the blood path is created and then excluded from the whole heart through boolean subtraction.

B) Cylindrical holes are created, as negative, in a copy of the resulting mask, and the 3D samples are isolated with boolean subtraction. Each volume is stored separately