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Development strategy of A. westerdijkiae ΔAwSakA strains. (A) Schematic diagram of AwSakA knockout strategy. (B) PCR validation of A. westerdijkiae WT and ΔAwSakA, WT (1) and three mutants ΔAwSakA-1 (2), ΔAwSakA-2 (3) and ΔAwSakA-3 (4) were identified by the paired primers of KO, UP and DOWN; M: 5kb maker.
