Figure 7.

The effect of different types of coercive agents on OTA biosynthesis in A. westerdijkiae WT and ΔAwSakA. (A) Production of OTA in WT and ΔAwSakA strains of A. westerdijkiae by D-sorbitol, NaCl, Congo red and H₂O₂. Different letters indicate significant differences between the corresponding values (p < 0.05) with three biological replicates. (B) Relative expression level of OTA biosynthetic genes of WT on media containing D-sorbitol, NaCl, Congo red and H₂O₂ analyzed by RT-qPCR. (C) The expression ratio (ΔAwSakA/WT) of OTA biosynthetic genes on media containing D-sorbitol, NaCl, Congo red and H₂O₂. Each treatment represents the mean of three independent experiments and the standard error is indicated by the bars.