TABLE 2.
Locations and sizes of ORFs in pTfars1b
| ORF | Position | No. of amino acids | Size (kDa) | Closest relationship to a known proteina | Product detectedb |
|---|---|---|---|---|---|
| ORF 1 | 1014-NDc | 338 | ND | 41% identity and 57% similarity to putative membrane protein of E. coli (accession no. ACC73882) | Yes |
| arsC | 1752-1261 | 163 | 18.1 | 53% identity and 65% similarity to P. aeruginosa ArsC (accession no. AAC69644) | Yes |
| arsR | 1752-2108 | 118 | 13.1 | 35% identity and 47% similarity to Mycobacterium tuberculosis hypothetical protein Rv2640c (accession no. CAB02346) | Yes |
| arsB | 2202-3512 | 436 | 48.4 | 57% identity and 70% similarity to ArsB from E. coli plasmid R773 (accession no. P08691) | Yes |
| arsH | 3518-4240 | 240 | 26.7 | 68% identity and 81% similarity to Y. enterocolitica ArsH (accession no. AAB42206) | Yes |
| ORF 5 | 4348-5724 | 458 | 44.7 | 58% identity and 72% similarity to signal recognition particle protein (fifty-four homologue) (P48) of E. coli (accession no. BAA16495) | Yes |
| ORF 6 | 5795-6055 | 86 | 9.56 | 66% identity and 79% similarity to 30S ribosomal protein S16 of Haemophilus influenzae Rd KW20 (accession no. P44382) | No |
| ORF 7 | 6111-6554 | 147 | 12.6 | 44% identity and 58% similarity to 16S rRNA-processing protein RIMM of Haemophilus influenzae Rd KW20 (accession no. P44568) | No |
| ORF 8 | 6554-ND | 94 | ND | 34% identity and 52% similarity to tRNA (guanine-N1)-methyltransferase of Salmonella typhimurium (accession no. P36245) | No |
a
The identity and similarity values are based on amino acid sequence data. The accession numbers are GenBank-EMBL database accession numbers.
b
Protein products were detected by using an in vitro E. coli transcription-translation system.
c
ND, not determined. The ORF was incomplete, and the molecular mass was not calculated; however, the number of amino acids is indicated.