Figure 3.

Knockdown of BAK function inhibits JEV-activated ROS and other cytokines. (A) DCFH-DA probes were loaded into normal and knockdown cells infected with JEV (MOI = 1, 48 hpi, experimental group) and controls. Fluorescence signals were collected at an excitation wavelength of 488 nm and emission wavelength of 525 nm, relative levels of intracellular ROS were calculated, and data were normalized and displayed as fold change (the data of the control group and the experimental group were normalized at the same time). (B) The relative mRNA levels of Caspase-1, NLRP3, IL-1β, and GSDMD in BAK.KD after JEV infection (MOI = 1, 48 hpi) were detected by real-time quantitative RT-PCR, and PK-15 cells were used as the control. * (p < 0.05), ** (p < 0.01), *** (p < 0.001), and ns (not significant).