Table 2.
Primers used for qRT-PCR.
| Gene | Sequence (5′-3′) | Length (bp) |
|---|---|---|
| JEV E | F: GGGAAGGGAAGCATTGAC R: AAGGAGCATTGGGTGTTA |
231 bp |
| BAK | F: TCAACCGGCGATACGACT R: GTAGCCAAAGCCCAGAAGAG |
155 bp |
| BAX | F: GCAGCCGATCTCGAAGGA R: GCCGAAATGTTTGCTGACG |
154 bp |
| IRF3 | F: AAGAAGCATTGCGTTTAGC R: AGGTACTGTATCTGCCATGAG |
138 bp |
| IRF7 | F: CATGGGGCGTGGATCTGA R: GCACCGTTCGACCTTTGT |
234 bp |
| TLR7 | F: TAACCTCAGTCAACCGCAAGT R: CCATCTTTGGGGCACATAC |
190 bp |
| TRANS | F: AGACGACTGCTGCCATGGA R: ACACCCTGCTGTTTTTCTACCT |
155 bp |
| RIG-1 | F: TCCCAGCAACGAGAACCCT R: TTCGTTTTGCCACGTCCAG |
195 bp |
| TNF | F: GGCATTGGCATACCCACTCT R: GCCCAAGGACTCAGATCATC |
178 bp |
| IFNα | F: GGACTCCATCCTGGCTGTGA R: GACTTCTGCCCTGATGATCTCC |
103 bp |
| IL-1β | F: ATGTGGACCTCTGGGTATGG R: ACAAAAGCCCGTCTTCCTG |
188 bp |
| IL-6 | F: GGCATCACCTTTGGCATCTT R: CTGCTTCTGGTGATGGTACTG |
204 bp |
| GSDMD | F: ACATGGCCTCCAAGGTAAGA R: GATCGAGTTGGGGCTGTGACT |
120 bp |
| NLRP3 | F: AATGGATGGGTTTACTGG R: GGTGAAGCGTTTGTTGAG |
189 bp |
| Caspase-1 | F: GTCCGAAGCGGTGAGATT R: CCCAGACATAGCCCAAAG |
220 bp |
| Caspase-3 | F: ACCCGAGTAAGAATGTGC R: GACTGTGGGATTGAGACG |
212 bp |
| Caspase-8 | F: CTCCTCCTCATTGGTTTCC R: TCCTGAGCCTGGACTACAT |
196 bp |
| GAPDH | F: ACATGGCCTCCAAGGTAAGA R: GATCGAGTTGGGGCTGTGACT |
202 bp |