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. 2023 Apr 20;6(2):43. doi: 10.3390/mps6020043

Figure 1.

Figure 1

Lysates containing RBD proteins run by traditional WB. (a) Image of membranes immunoblotted with anti-SARS-CoV-2 RBD (1:2500) or anti-GAPDH (1:1000) Abs using traditional WB. (b) Floating box plots showing the minimum, maximum, and mean of the band volumes for RBD and GAPDH bands for RBD1–4 samples from three independent experiments that were obtained using Image Lab analysis. Lysates (10, 2.5, and 1 µg/lane) were from 293T cells transfected with plasmids expressing different-sized transgenes containing RBD (RBD1–4) or control transgene (CTRL). MW: PageRuler™ Plus Prestained Protein Ladder (Thermo Scientific).