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. 2023 Apr 28;42:106. doi: 10.1186/s13046-023-02655-8

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Recovered mice reject tumor in rechallenge experiment. A Left/middle: Tumor measurements of tumor-ablating BALB/c (white) and C57BL/6 (black) mice rechallenged with the same number of 4T1 or B16-F10 cancer cells, respectively, on either day 60 or 70 after the first tumor injection. Right: Tumor measurements of 4T1 tumor-ablating mice rechallenged with B16-F10 cancer cells on day 47 after the first tumor injection. Naïve mice were included in all rechallenges as controls for tumor take. B + C Flow cytometry on cell populations from spleens and LNs from either naïve mice (blue), or mice that had recovered from 4T1 or CT26 cancer (red). The percentage of cells represents the percentage of live cells within the lymphocyte gate. Differences between cell fractions in naive and survivor mice were evaluated using the Mann–Whitney test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001