Fig. 2.

Osteosarcoma cells adopt distinct transcriptional profiles as they colonize tibia and lung microenvironments. A Schematic of study design depicting generation of orthotopic tibia- and lung-colonizing tumors. Tumors were harvested for scRNA-seq when mice reached endpoint. B Venn diagrams showing overlap of differentially expressed genes that are up- or downregulated in tibia or lung lesions relative to corresponding starting population of cells (cell culture or PDX flank tumors). Regions outlined in red identify differentially expressed genes shared across at least three models. C Pathway enrichment analysis with adjusted p values for hallmark gene sets associated with these shared genes