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Purification of CODH from B. japonicum 110spc4
| Step | Protein (mg) | Total activity (nmol of CO oxidized/min) | Sp act (nmol of CO oxidized/min/mg of protein) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
| Cytoplasmic fraction | 1,296 | 3,583 | 2.8 | 1 | 100 |
| Anion-exchange chromatography | 280 | 2,423 | 8.6 | 3.1 | 67.6 |
| Hydrophobic interaction chromatography | 24 | 630 | 26 | 9.3 | 17.6 |
| Gel filtration | 9.6 | 554 | 57.7 | 20.6 | 15.5 |
