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. 2023 Apr 18;11(4):864. doi: 10.3390/vaccines11040864

Figure 3.

Figure 3

Immunization with recombinant proteins induces specific IgM (A–C) and IgG (D–F) anti-SARS-CoV-2 N, S1, and RBD antibodies, as evaluated using ELISAs. Sera were diluted to 1:200, and the results are expressed as O.D. at 450 nm, and the data of each group of 10 mice are plotted. For more than two samples, nonparametric ANOVA was used; bars represent the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. p ≤ 0.05 was considered statistically significant. Prism v.8 software was used for statistical analyses (GraphPad Inc., La Jolla, CA, USA).