Figure 6.

Immunization with recombinant proteins elicits a cellular response. Splenocytes from mice immunized with recombinant proteins alone or in combination were stimulated with 2 μg/mL of N, S1, and RBD recombinant proteins. No stimulated cells were used as controls. After 48 h, the levels of IFN-γ, TNF-α, IL-2, IL-4, and IL-5 were determined using CBAs in the supernatants of these cells. Each point represents a pool of three mice, and each bar represents an average of a group of 3 mice. A nonparametric ANOVA test was used; bars represent the mean ± SD. *** p < 0.001. p ≤ 0.05 was considered statistically significant. Prism v.8 software was used for statistical analyses (GraphPad Inc., La Jolla, CA, USA).