Figure 7.

Recognition of RBD variants by sera from immunized mice. IgG antibodies against RBD Wuhan, Alpha, and Beta were evaluated using ELISA. Sera were diluted to 1:200. IgG antibodies were observed in response to RBD variants expressed in the E. coli system. Lower levels of these antibodies were detected in the variant groups than in the RBD Wuhan group, although the difference was not statistically significant. Bars represent the mean ± SD. p ≤ 0.05 was considered statistically significant. The Wilcoxon test in Prism v.8 software was used for statistical analyses (GraphPad Inc., La Jolla, CA, USA).