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. 2023 Apr 19;15(4):298. doi: 10.3390/toxins15040298

Figure 3.

Figure 3

Effects of BNP on LPS-induced inflammatory cytokines by ELISA assay. Levels of (a) TNF-α, (b) IL-6 and (c) IL-10, and (d) TNF-α/IL-10 ratio after BNP treatment of LPS-activated RAW 264.7 cells. After LPS (1 µg/mL) priming for 24 h, cells were treated with different concentrations of BNP (0, 0.05, 0.1, 0.2, 0.4, 0.8, 1.6, 3.2 and 6.4 ng/mL) for 24 h to determine TNF-α and IL-6, and for 48 h to assess IL-10 levels. Natriuretic peptide receptor antagonist isatin (0.1 mM) was added 20 min before BNP treatment. n = 3–6/condition. Data are reported as mean ± SEM. *** p < 0.001 vs. corresponding control LPS untreated group; $ p < 0.05, $$ p < 0.01 vs. corresponding control BNP untreated group; # p < 0.05, ### p < 0.001 vs. corresponding LPS + BNP treated group.