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. 2023 Apr 17;15(4):1262. doi: 10.3390/pharmaceutics15041262

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Ex vivo characterization of medical devices for prospective vaccine delivery using PBAE polyelectrolyte films. Capacity of transdermal devices to effectively penetrate the skin was tested using cadaveric skin explants from C57BL/6 healthy mice along with film deposition from rods (top panel) or solid PLGA-based MNs (bottom panel) (A). Fluorescence microscopy following transfection with the K/D and K/D-Man polymers as PBAE:pDNA polyplexes (top) and PBAE:pDNA films (bottom) in HaCaT cells (B). Scale bar = 50 µm.