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(A) Schematic representation of the working electrode modifications: (I) electrogeneration of the AuNPs using a solution of tetrachloroauric acid and subsequent reduction of Au(III) by applying a potential equal to +0.18 V to the working electrode for 50 s, (II) immobilization of tyrosinase enzyme (100 IU/μL), (III) cross-linking reaction using a 25% gluteraldehyde solution. (B) The oxidation process of phenol to ortho-quinone catalyzed by tyrosinase.
