Figure 3.

The DV2 peptide inhibits ZIKV infectivity in vitro cultured Vero cells. Vero cell monolayers were infected with ZIKV (MOI = 1.0) in the presence of the DV2 (DV2) or Ph22 (Control) peptides. The impact of the treatment with the DV2 peptide on virus infection was measured at 24 hpi. (a) Percentages of infected cells determined by flow cytometry. (b) Viable ZIKV titers (PFU/mL) determined by plaque assay using culture supernatants of infected cells. Cells incubated with viruses treated with Ph22, DMSO, or culture medium (NT) were used as negative controls. The data are presented as mean ± SEM (bars) from three independent experiments. Statistically significant differences were determined using two-way ANOVA with Bonferroni correction (*** p < 0.001 and **** p < 0.0001, compared to the DMSO control).