FIG. 2.

Radiochromatograms of culture fluids from 24-hr incubations of [¹⁴C]pyrene with P. saccharophila P15 (a), P. stutzeri P16 (b), B. cereus P21 (c), and S. yanoikuyae R1 (d). The inset to panel a is the chromatogram from incubation of strain P15 with [¹⁴C]pyrene in the presence of 50 μM PYRdHD for 24 h, at which time most of the PYRdHD was depleted; the chromatogram is truncated before the much larger peak for unreacted pyrene. Solid lines are sample chromatograms, and dashed lines are chromatograms from uninoculated controls. PYRdHD, PYRQ, and pyrene eluted at 14, 19, and 29 min, respectively, under the HPLC conditions used to generate these chromatograms.