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. 2023 Mar 30;21(4):223. doi: 10.3390/md21040223

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Anti-inflammatory assays of compounds 1–7. (a) Images of inflammatory sites in CuSO₄-induced transgenic fluorescent zebrafish (Tg: zlyz-EGFP) expressing enhanced green fluorescent protein (EGFP) treated with compounds 1–7, using indomethacin as a positive control. (b) Quantitative analysis of macrophages in the region of inflammatory sites in zebrafish treated with compounds 1–7 at 20 μM. #### indicates that the CuSO₄ model group has a very significant difference compared with the control group (p < 0.01). ** indicates that the sample groups have significant differences compared with the CuSO₄ model group (p < 0.01).