Table 1.

The results of the purification of AH from Y. lipolytica cells of the studied groups *.

Stage of Purification	Group	Total Activity, Units	The Protein Amount, mg	Specific Activity, E/mg Protein	Yield, %	Degree of Purification
Homogenate	1 (pH 4.0)	2.25 ± 0.101	250 ± 11.5	0.009 ± 0.0003	100	1
	2 (pH 5.5)	2.79 ± 0.112 a,b	233 ± 10.8 a	0.012 ± 0.0003 a	100	1
	3 (pH 9.0)	1.94 ± 0.096 b	277 ± 12.3 b	0.007 ± 0.0002 b	100	1
Chromatography on sephadex G-25	1 (pH 4.0)	1.77 ± 0.082	183 ± 9.2	0.015 ± 0.0006	79	1.7
	2 (pH 5.5)	2.29 ± 0.111 a,c	111 ± 5.2 a	0.018 ± 0.0007 a	82	1.5
	3 (pH 9.0)	1.43 ± 0.061	182 ± 8.5	0.012 ± 0.0006	74	1.7
Chromatography on DEAE cellulose	1 (pH 4.0)	0.765 ± 0.034 c	1.77 ± 0.08 c	0.432 ± 0.0212 c	34	48
	2 (pH 5.5)	0.865 ± 0.042 d	1.57 ± 0.07	0.552 ± 0.0271	31	46
	3 (pH 9.0)	0.357 ± 0.015 d	1.58 ± 0.07	0.357 ± 0.0158 d	29	51

* Note: Table discusses statistically significant differences at: ^a—p < 0.01; ^b—p < 0.0004; ^c—p < 0.03; ^d—p < 0.003.