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. 2023 Apr 21;24(8):7670. doi: 10.3390/ijms24087670

Table 2.

Kinetic parameters of the catalytic action of AH from Y. lipolytica when grown under conditions of different pH values *.

Growth Conditions Citrate Isocitrate pH-Optimum
Vmax, mmols/mg of Protein- Km, mM Vmax, mmols/mg of Protein- Km, mM
pH 4.0 0.570 ± 0.042 a 0.230 ± 0.009 b,c 0.415 ± 0.017 a 0.150 ± 0.008 c 7.6
pH 5.5 0.720 ± 0.031 c 0.320 ± 0.011 0.726 ± 0.037 c 0.270 ± 0.015 b,c 8.0
pH 9.0 0.428 ± 0.022 a 0.190 ± 0.007 b 0.394 ± 0.016 a 0.140 ± 0.006 c 8.2

* Note: The table discusses statistically significant differences at p ≤ 0.05. ap < 0.004; bp < 0.003; cp < 0.001. Values are mean ± standard deviation from 5–6 independent experiments.