Table 2.
Kinetic parameters of the catalytic action of AH from Y. lipolytica when grown under conditions of different pH values *.
Growth Conditions | Citrate | Isocitrate | pH-Optimum | ||
---|---|---|---|---|---|
Vmax, mmols/mg of Protein- | Km, mM | Vmax, mmols/mg of Protein- | Km, mM | ||
pH 4.0 | 0.570 ± 0.042 a | 0.230 ± 0.009 b,c | 0.415 ± 0.017 a | 0.150 ± 0.008 c | 7.6 |
pH 5.5 | 0.720 ± 0.031 c | 0.320 ± 0.011 | 0.726 ± 0.037 c | 0.270 ± 0.015 b,c | 8.0 |
pH 9.0 | 0.428 ± 0.022 a | 0.190 ± 0.007 b | 0.394 ± 0.016 a | 0.140 ± 0.006 c | 8.2 |
* Note: The table discusses statistically significant differences at p ≤ 0.05. a—p < 0.004; b—p < 0.003; c—p < 0.001. Values are mean ± standard deviation from 5–6 independent experiments.