TABLE 1.

Effects of various compounds on the production of half-amidase^a

Compound	Mean ± SD		Relative activity (%)
	Optical density at 610 nm	Sp act (μmol/min/mg of protein)
Expt 1
Additives in sucrose-containing medium
None	0.335 ± 0.012	0.176 ± 0.018	100
Succinimide	0.250 ± 0.012	0.325 ± 0.010	185
Glutarimide	0.392 ± 0.011	0.476 ± 0.008	270
Succinamic acid	0.314 ± 0.011	0.163 ± 0.016	93
Adipinamic acid	0.323 ± 0.014	0.150 ± 0.008	85
Succinic acid	0.414 ± 0.005	0.300 ± 0.015	170
Adipic acid	0.355 ± 0.018	0.188 ± 0.008	107
Acetamide	0.223 ± 0.011	0.176 ± 0.015	100
n-Valeramide	0.267 ± 0.006	0.213 ± 0.012	121
Lactamide	0.222 ± 0.013	0.178 ± 0.005	99
Benzamide	0.140 ± 0.010	Traceb	NDc
Dihydrouracil	0.330 ± 0.019	0.213 ± 0.010	121
Dihydrothymine	0.202 ± 0.004	0.159 ± 0.013	90
Ammonium chloride	0.236 ± 0.010	0.201 ± 0.005	114
l-Glutamine	1.100 ± 0.016	0.240 ± 0.004	135
Expt 2
Additives as sole carbon sources
Succinimide	0.233 ± 0.010	0.582 ± 0.013	331
Glutarimide	0.101 ± 0.006	0.960 ± 0.016	545

^aThe standard deviations were obtained from four separate determinations. The methods for experiments 1 and 2 are described in Materials and Methods. 

^bLess than the specific activity of 10⁻³ μmol/min/mg of protein. 

^cND, not determined.