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Purification of half-amidase from Blastobacter sp. strain A17p-4
| Step | Vol (ml) | Total protein (mg) | Total activity (μmol/min) | Sp act (μmol/min/mg) | Recovery (%) | Fold |
|---|---|---|---|---|---|---|
| Cell extract | 230 | 1,890 | 12.4 | 0.00656 | 100 | 1.00 |
| DEAE-Sephacel | 90 | 114 | 11.0 | 0.0965 | 88.7 | 14.7 |
| Phenyl-Sepharose | 65 | 52.7 | 3.10 | 0.0588 | 25.0 | 8.96 |
| Sephacryl S-200 | 40 | 7.00 | 1.01 | 0.144 | 8.15 | 22.0 |
| Mono Q HR 5/5 | 2.0 | 0.103 | 0.209 | 2.03 | 1.69 | 309 |
| Mono Q PC 1.6/5 | 0.2 | 0.0221 | 0.111 | 5.02 | 0.895 | 765 |
