Table 3.
Thermodynamic parameters obtained from the ITC binding data for the interactions of wtSSB-Ct variants with ExoI and RecO. Confidence intervals are shown for KD and ΔH values.
| ExoI | RecO | |||||
|---|---|---|---|---|---|---|
| wtSSB-Ct | E1-sSSB-Ct | E2-sSSB-Ct | wtSSB-Ct | R1-sSSB-Ct | R2-sSSB-Ct | |
| N | 0.9593 | 1.0000 | 0.8846 | 1.0032 | 0.9353 | 0.9999 |
| KD (µM) | 3.07 (2.62–3.64) |
0.02 (0.01–0.03) |
0.04 (0.02–0.07) |
0.14 (0.08–0.24) |
0.13 (0.08–0.22) |
0.04 (0.02–0.05) |
| ΔG (kcal/mol) | −7.78 | −10.89 | −10.43 | −9.77 | −9.71 | −10.54 |
| ΔH (kcal/mol) | −10.67 (−11.64–−9.97) |
−17.35 (−18.34–−16.43) |
−13.45 (−14.46–−12.49) |
−7.54 (−8.28–−6.90) |
−6.79 (−7.84–−6.05) |
−23.48 (−24.34–−22.73) |
| −TΔS (kcal/mol) | 2.90 | 6.46 | 3.02 | −2.28 | −2.91 | 12.32 |